To compare cell types across organs, up to 800 cells were randomly sampled per cell type per tissue (or in cases where less than 800 cells of a given cell type were represented in a given tissue, all cells were taken). Seurat object contains peak by cell matrix for 86,685 cells and 1,001,437 peaks (z-score filtered master list) in the peaks assay and the gene by cell matrix in the RNA assay.
Cell metadata for all cells, including tissue of origin, donor ID, estimated gestational age, sex, experimental batch, total number of reads, total number of deduplicated reads, total number of deduplicated reads that fall into peaks, total number of deduplicated reads that fall into a 2 kb window centered on TSS, fraction of reads in peaks (frip), fraction of reads in 2 kb window centered on TSS (frit), number of peaks that contain at least one read (nFeature_peaks), Louvain cluster ID and UMAP coordinates of per tissue (not combined) UMAP visualizations and cell type annotation.
Motif enrichment across cell types
For each of the 579 motifs from the JASPAR vertebrate database, the enrichment in accessible sites in each of the main 54 cell types was determined using a linear regression model. Fold-change of the mean motif occurrence in sites of a given cell type relative to the rest of the dataset and matching Benjamini Hochberg-adjusted p-values are reported for each motif-cell type pair.